Deep Sequencing of the Human TCR and TCR Repertoires Suggests that TCR Rearranges After and T Cell Commitment

Stoichiometry of the T cell Antigen Receptor ( TCR ) Complex : Each TCR / CD 3 Complex Contains One TCR ol , One TCR [

The stoichiometry of the subunits that comprise the T cell antigen receptor (TCR) complex is not completely known. In particular, it is uncertain whether TCR ot and TCR/5 proteins are present in the TCR complex as one or multiple heterodimeric pairs. In this study we have used mice transgenic for two different TCR ot and two different TCR/5 proteins to determine the number of TCR a and TCR/5 ch...

Homogenization of TCR Repertoires within

Immunotherapy with TCR-redirected T cells: comparison of TCR-transduced and TCR-engineered hematopoietic stem cell-derived T cells.

Redirecting Ag specificity by transfer of TCR genes into PBLs is an attractive method to generate large numbers of cytotoxic T cells for immunotherapy of cancer and viral diseases. However, transferred TCR chains can pair with endogenous TCR chains, resulting in the formation of mispaired TCR dimers and decreased or unspecific reactivity. TCR gene transfer into hematopoietic stem cells (HSCs) i...

Huge Overlap of Individual TCR Beta Repertoires

It has been reported that human TCR repertoires commonly carry so-called public clonotypes – CDR3 variants that are often shared between individuals. Crosscomparison of individual immune repertoires has previously revealed the existence of a population of TCR beta CDR3 variants that are identical at the amino acid level for any two donors (1–3). The lower bound for the total overlap between any...

Re-shaping the T cell repertoire: TCR editing and TCR revision for good and for bad.

Protection against the universe of pathogens requires a functional, diverse T cell repertoire. However, the price that is paid for an evolved, effective immune system includes the potential danger of generating autoaggressive T cells. Autoimmune diseases result from inherent breach of tolerance to self-antigens leading to disruption of the regulatory to autoaggressive T cell homeostatic balance...